Journal: EMBO Reports

Article Title: Proneurotrophin-3 contributes to chemotherapy-induced neuropathic pain through TrkC-mediated CCL2 elevation in DRG neurons

doi: 10.1038/s44319-025-00534-1

Figure Lengend Snippet: Data: mean ± SD. ( A , B ) Intraperitoneal injection of paclitaxel produced mechanical allodynia as evidenced by the increases in paw withdrawal frequencies in response to 0.07 g ( A ) and 0.4 g ( B ) von Frey filaments on the left side. n = 12 mice/time point. * P < 0.05, ** P < 0.01 versus the vehicle-treated group at the corresponding time points by two-way ANOVA with repeated measures followed by post hoc Tukey test. ( C , D ) The levels of Nt3 mRNA ( C ) and proNT3 protein ( D ) in L3/4 DRGs on different days as indicated after the first injection of paclitaxel or vehicle. Representative Western blots (left) and a summary of the densitometric analysis (right) are shown. n = 3 biological repeats (3 mice)/time point. * P < 0.05, ** P < 0.01 by two-way ANOVA followed by post hoc Tukey test. ( E ) The amount of proNT3 protein in the L3/4 spinal cord on different days as indicated after the first injection of paclitaxel. n = 3 biological repeated (3 mice)/time point. P > 0.05 by one-way ANOVA followed by post hoc Tukey test. .

Article Snippet: Figure EV1 Expression of proNT3, but not NT3, is detected in the DRG of naive and paclitaxel-treated mice. ( A ) Total cellular lysates from the DRG of naive mice and recombinant NT3 standard (SinoBiological) are Western blotted with an anti-NT3 antibody (ANT-003, Alomone Labs) to demonstrate that this antibody recognizes both pro-NT3 and NT3. proNT3 (~32 kDa), but not NT3 ( ~ 14.5 kDa), is detected in DRG lysate from naive mice. ( B ) pro-NT3 is highly expressed in the DRG, spinal cord, cortex, cerebellum and brainstem.

Techniques: Injection, Produced, Western Blot

Journal: EMBO Reports

Article Title: Proneurotrophin-3 contributes to chemotherapy-induced neuropathic pain through TrkC-mediated CCL2 elevation in DRG neurons

doi: 10.1038/s44319-025-00534-1

Figure Lengend Snippet: ( A ) Total cellular lysates from the DRG of naive mice and recombinant NT3 standard (SinoBiological) are Western blotted with an anti-NT3 antibody (ANT-003, Alomone Labs) to demonstrate that this antibody recognizes both pro-NT3 and NT3. proNT3 (~32 kDa), but not NT3 ( ~ 14.5 kDa), is detected in DRG lysate from naive mice. ( B ) pro-NT3 is highly expressed in the DRG, spinal cord, cortex, cerebellum and brainstem. In contrast, NT3 is undetected in the DRG and expressed weakly in the spinal cord, cortex, cerebellum and brainstem. ( C ) Expression of proNT3 is time-dependently increased in the DRG after first intraperitoneal (i.p.) injection of paclitaxel. NT3 is not detected in the DRG on days 0, 7, 10, 14, 21, and 28 after the first i.p. paclitaxel injection. M: Molecular weight marker. .

Article Snippet: Figure EV1 Expression of proNT3, but not NT3, is detected in the DRG of naive and paclitaxel-treated mice. ( A ) Total cellular lysates from the DRG of naive mice and recombinant NT3 standard (SinoBiological) are Western blotted with an anti-NT3 antibody (ANT-003, Alomone Labs) to demonstrate that this antibody recognizes both pro-NT3 and NT3. proNT3 (~32 kDa), but not NT3 ( ~ 14.5 kDa), is detected in DRG lysate from naive mice. ( B ) pro-NT3 is highly expressed in the DRG, spinal cord, cortex, cerebellum and brainstem.

Techniques: Recombinant, Western Blot, Expressing, Injection, Molecular Weight, Marker

Journal: EMBO Reports

Article Title: Proneurotrophin-3 contributes to chemotherapy-induced neuropathic pain through TrkC-mediated CCL2 elevation in DRG neurons

doi: 10.1038/s44319-025-00534-1

Figure Lengend Snippet: Data: mean ± SD. ( A , B ) Representative images of in situ hybridization histochemistry (ISHH) for Nt3 mRNA (red) and immunohistochemistry for β-tubulin III (green; A ) or glutamine synthetase (GS; green; B ) in the L3/4 DRGs 14 days after the first paclitaxel or vehicle injection. Cellular nuclei were labeled by 4’, 6-diamidino-2-phenylindole (DAPI; blue). n = 9–12 sections from three mice/group. * P < 0.05 by two-tailed unpaired Student t test. Scale bars: 50 µm. ( C ) Size distribution of Nt3 mRNA-labeled neuronal somata in the L3/4 DRGs 14 days after the first paclitaxel injection. Small, 25%, medium, 36%; large, 39%. ( D – H ) Representative images of ISHH for Nt3 mRNA (red) and immunohistochemistry for different DRG cell markers (calcitonin gene-related peptide (CGRP; green; D ), neurofilament 200 (NF200; green; E ), isolectin B4 (IB4; green; F ), or tyrosine hydroxylase (TH; green; G )) in the L3/4 DRGs 14 days after the first paclitaxel injection. Arrows: double-labeling neurons. Statistical summary of number of double-labeling neurons ( H ). n = 9–12 sections from 3 mice. Scale bar: 50 µm. .

Article Snippet: Figure EV1 Expression of proNT3, but not NT3, is detected in the DRG of naive and paclitaxel-treated mice. ( A ) Total cellular lysates from the DRG of naive mice and recombinant NT3 standard (SinoBiological) are Western blotted with an anti-NT3 antibody (ANT-003, Alomone Labs) to demonstrate that this antibody recognizes both pro-NT3 and NT3. proNT3 (~32 kDa), but not NT3 ( ~ 14.5 kDa), is detected in DRG lysate from naive mice. ( B ) pro-NT3 is highly expressed in the DRG, spinal cord, cortex, cerebellum and brainstem.

Techniques: In Situ Hybridization, Immunohistochemistry, Injection, Labeling, Two Tailed Test

Journal: EMBO Reports

Article Title: Proneurotrophin-3 contributes to chemotherapy-induced neuropathic pain through TrkC-mediated CCL2 elevation in DRG neurons

doi: 10.1038/s44319-025-00534-1

Figure Lengend Snippet: Data: mean ± SD. ( A ) Expression of proNT3 protein in the ipsilateral L3/4 DRGs 14 days after the first injection of paclitaxel (PTX) or vehicle (Veh) from the NT3 siRNA- or NC siRNA-microinjected male mice. n = 3 biological repeats (6 mice)/group. * P < 0.05, ** P < 0.01 by two-way ANOVA followed by post hoc Tukey test. ( B – H ) Paw withdrawal frequency to 0.07 g ( B , F ) and 0.4 g ( C, G ) von Frey filament stimuli and paw withdrawal latency to heat ( D, H ) and cold ( E ) stimuli on the ipsilateral ( B – E ) and contralateral ( F–H ) side on days as indicated from male mice with DRG microinjection of NT3 siRNA or NC siRNA 7 days after the first injection of paclitaxel (PTX) or vehicle. n = 9 mice/group. * P < 0.05, ** P < 0.01 versus the paclitaxel plus NC siRNA-treated group at the corresponding time points by three-way ANOVA with repeated measures followed by post hoc Tukey test. ( I ) Levels of p-ERK1/2, total ERK1/2, and GFAP in the ipsilateral L3/4 dorsal horn 14 days after the first injection of paclitaxel (PTX) or vehicle (Veh) from male mice with DRG microinjection of NT3 siRNA or NC siRNA. n = 3 mice/group. * P < 0.05, ** P < 0.01 by two-way ANOVA followed by Tukey post hoc test. .

Article Snippet: Figure EV1 Expression of proNT3, but not NT3, is detected in the DRG of naive and paclitaxel-treated mice. ( A ) Total cellular lysates from the DRG of naive mice and recombinant NT3 standard (SinoBiological) are Western blotted with an anti-NT3 antibody (ANT-003, Alomone Labs) to demonstrate that this antibody recognizes both pro-NT3 and NT3. proNT3 (~32 kDa), but not NT3 ( ~ 14.5 kDa), is detected in DRG lysate from naive mice. ( B ) pro-NT3 is highly expressed in the DRG, spinal cord, cortex, cerebellum and brainstem.

Techniques: Expressing, Injection, Microinjection

Journal: EMBO Reports

Article Title: Proneurotrophin-3 contributes to chemotherapy-induced neuropathic pain through TrkC-mediated CCL2 elevation in DRG neurons

doi: 10.1038/s44319-025-00534-1

Figure Lengend Snippet: Data: mean ± SD. ( A ) Expression of proNT3 protein in the ipsilateral L3/4 DRGs 14 days after the first injection of paclitaxel (PTX) or vehicle (Veh) from the NT3 siRNA- or NC siRNA-microinjected female mice. n = 3 biological repeats (6 mice)/group. * P < 0.05, ** P < 0.01 by two-way ANOVA followed by post hoc Tukey test. ( B – H ) Paw withdrawal frequency to 0.07 g ( B , F ) and 0.4 g ( C , G ) von Frey filament stimuli and paw withdrawal latency to heat ( D , H ) and cold ( E ) stimuli on the ipsilateral ( B – E ) and contralateral ( F – H ) side on the days as indicated from female mice with DRG microinjection of NT3 siRNA or NC siRNA on day 7 after the first injection of paclitaxel or vehicle. n = 9 mice/group. * P < 0.05, ** P < 0.01 versus the paclitaxel plus NC siRNA-treated group at the corresponding time points by three-way ANOVA with repeated measures followed by post hoc Tukey test. ( I ) Levels of p-ERK1/2, total ERK1/2, and GFAP in the ipsilateral L3/4 dorsal horn 14 days after the first injection of paclitaxel (PTX) or vehicle (Veh) from female mice with DRG microinjection of NT3 siRNA or NC siRNA. n = 3 mice/group. ** P < 0.01 by two-way ANOVA followed by Tukey post hoc test. .

Article Snippet: Figure EV1 Expression of proNT3, but not NT3, is detected in the DRG of naive and paclitaxel-treated mice. ( A ) Total cellular lysates from the DRG of naive mice and recombinant NT3 standard (SinoBiological) are Western blotted with an anti-NT3 antibody (ANT-003, Alomone Labs) to demonstrate that this antibody recognizes both pro-NT3 and NT3. proNT3 (~32 kDa), but not NT3 ( ~ 14.5 kDa), is detected in DRG lysate from naive mice. ( B ) pro-NT3 is highly expressed in the DRG, spinal cord, cortex, cerebellum and brainstem.

Techniques: Expressing, Injection, Microinjection

Journal: EMBO Reports

Article Title: Proneurotrophin-3 contributes to chemotherapy-induced neuropathic pain through TrkC-mediated CCL2 elevation in DRG neurons

doi: 10.1038/s44319-025-00534-1

Figure Lengend Snippet: Data: mean ± SD. ( A , B ) Levels of Nt3 mRNA ( A ) and proNT3 protein ( B ) in the L3/4 DRGs 28 days after the first intraperitoneal (i.p.) injection of paclitaxel (PTX) or vehicle (Veh) from the tamoxifen-treated NT3 f/f mice and NT3 cKD mice. n = 3 biological repeated (3 mice)/group. * P < 0.05, ** P < 0.01 by two-way ANOVA followed by post hoc Tukey test. ( C-I ) Paw withdrawal frequency to 0.07 g ( C , G ) and 0.4 g ( D , H ) von Frey filament stimuli and paw withdrawal latency to heat ( E , I ) and cold ( F ) stimuli on the left ( C – F ) and right ( G – I ) side on days as indicated from NT3 f/f mice and NT3 cKD with i.p. injection of tamoxifen (Tam) daily for 7 consecutive days starting at day 7 after the first i.p. injection of paclitaxel (PTX) or vehicle. n = 9 mice/group. * P < 0.05, ** P < 0.01 versus the paclitaxel-treated NT3 f/f mice at the corresponding days by three-way ANOVA with repeated measures followed by post hoc Tukey test. ( J , K ) Spontaneous ongoing pain as assessed by the CPP test 21 days after the first i.p. injection of paclitaxel (PTX) or vehicle in the tamoxifen-treated NT3 f/f mice and NT3 cKD mice. Time spent in each chamber ( J ) and difference scores for chamber preferences calculated by subtracting preconditioning (Pre) preference time from postconditioning (Post) time spent in the lidocaine-paired chamber ( K ). n = 9 mice/group. ** P < 0.01 by three-way ANOVA with repeated measures followed by post hoc Tukey test ( J ) or by two-way ANOVA followed by Tukey post hoc test ( K ). ( L ) Levels of p-ERK1/2, total ERK1/2 and GFAP in the L3/4 dorsal horn 28 days after the first i.p. injection of paclitaxel (PTX) or vehicle in the tamoxifen-treated NT3 f/f mice and NT3 cKD mice. n = 3 mice/group. * P < 0.05, ** P < 0 . 01 by two-way ANOVA followed by post hoc Tukey test. .

Article Snippet: Figure EV1 Expression of proNT3, but not NT3, is detected in the DRG of naive and paclitaxel-treated mice. ( A ) Total cellular lysates from the DRG of naive mice and recombinant NT3 standard (SinoBiological) are Western blotted with an anti-NT3 antibody (ANT-003, Alomone Labs) to demonstrate that this antibody recognizes both pro-NT3 and NT3. proNT3 (~32 kDa), but not NT3 ( ~ 14.5 kDa), is detected in DRG lysate from naive mice. ( B ) pro-NT3 is highly expressed in the DRG, spinal cord, cortex, cerebellum and brainstem.

Techniques: Injection

Journal: EMBO Reports

Article Title: Proneurotrophin-3 contributes to chemotherapy-induced neuropathic pain through TrkC-mediated CCL2 elevation in DRG neurons

doi: 10.1038/s44319-025-00534-1

Figure Lengend Snippet: Data: mean ± SD. ( A , B ) Level of Nt3 mRNA ( A ) and proNT3 protein ( B ) in the ipsilateral L3/4 DRGs 8 weeks after microinjection of AAV5-NT3 or control AAV5-GFP in male mice. n = 3 biological repeats (6 mice)/group. ** P < 0.01 by two-tailed unpaired Student t test. ( C – F ) Paw withdrawal frequency to 0.07 g ( C ) and 0.4 g ( D ) von Frey filament stimuli and paw withdrawal latency to heat ( E ) and cold ( F ) stimuli on the ipsilateral and contralateral sides on weeks as indicated after microinjection of AAV5-NT3 or AAV5-GFP into unilateral L3/4 DRGs. n = 9 mice/group. * P < 0.05, ** P < 0.01 versus the AAV5-GFP group on the ipsilateral side at the corresponding time points by three-way ANOVA with repeated measures followed by Tukey post hoc test. ( G , H ) Spontaneous ongoing pain as assessed by the CPP test 8 weeks after microinjection of AAV5-NT3 or AAV5-GFP into unilateral L3/4 DRGs. Time spent in each chamber ( G ) and difference scores for chamber preferences calculated by subtracting preconditioning (Pre) preference time from postconditioning (Post) time spent in the lidocaine-paired chamber ( H ). n = 9 mice/group. ** P < 0.01 by two-way ANOVA with repeated measures followed by post hoc Tukey test ( G ) or by two-tailed unpaired Student t test ( H ). ( I ) Levels of p-ERK1/2, total ERK1/2, and GFAP in the ipsilateral L3/4 dorsal horn 8 weeks after microinjection of AAV5-NT3 or AAV5-GFP into unilateral L3/4 DRGs. n = 3 mice/group. ** P < 0.01 by two-tailed unpaired Student t test. .

Article Snippet: Figure EV1 Expression of proNT3, but not NT3, is detected in the DRG of naive and paclitaxel-treated mice. ( A ) Total cellular lysates from the DRG of naive mice and recombinant NT3 standard (SinoBiological) are Western blotted with an anti-NT3 antibody (ANT-003, Alomone Labs) to demonstrate that this antibody recognizes both pro-NT3 and NT3. proNT3 (~32 kDa), but not NT3 ( ~ 14.5 kDa), is detected in DRG lysate from naive mice. ( B ) pro-NT3 is highly expressed in the DRG, spinal cord, cortex, cerebellum and brainstem.

Techniques: Microinjection, Control, Two Tailed Test

Journal: EMBO Reports

Article Title: Proneurotrophin-3 contributes to chemotherapy-induced neuropathic pain through TrkC-mediated CCL2 elevation in DRG neurons

doi: 10.1038/s44319-025-00534-1

Figure Lengend Snippet: Data: mean ± SD. ( A , B ) Level of Nt3 mRNA ( A ) and proNT3 protein ( B ) in the ipsilateral L3/4 DRGs 8 weeks after microinjection of AAV5-NT3 or control AAV5-GFP in female mice. n = 3 biological repeats (6 mice)/group. ** P < 0.01 by two-tailed unpaired Student t test. ( C – F ) Paw withdrawal frequency to 0.07 g ( C ) and 0.4 g ( D ) von Frey filament stimuli and paw withdrawal latency to heat ( E ) and cold ( F ) stimuli on the ipsilateral and contralateral sides in weeks as indicated after microinjection of AAV5-NT3 or AAV5-GFP into unilateral L3/4 DRGs. n = 9 mice/group. * P < 0.05, ** P < 0.01 versus the AAV5-GFP group on the ipsilateral side at the corresponding time points by three-way ANOVA with repeated measures followed by Tukey post hoc test. ( G , H ) Spontaneous ongoing pain as assessed by the CPP test 8 weeks after microinjection of AAV5-NT3 or AAV5-GFP into unilateral L3/4 DRGs. Time spent in each chamber ( G ) and difference scores for chamber preferences calculated by subtracting preconditioning (Pre) preference time from postconditioning (Post) time spent in the lidocaine-paired chamber ( H ). n = 9 mice/group. ** P < 0.01 by two-way ANOVA with repeated measures followed by post hoc Tukey test ( J ) or by two-tailed unpaired Student t test ( H ). ( I ) Levels of p-ERK1/2, total ERK1/2, and GFAP in the ipsilateral L3/4 dorsal horn 8 weeks after microinjection of AAV5-NT3 or AAV5-GFP into unilateral L3/4 DRGs. n = 3 mice/group. ** P < 0.01 by two-tailed unpaired Student t test. .

Article Snippet: Figure EV1 Expression of proNT3, but not NT3, is detected in the DRG of naive and paclitaxel-treated mice. ( A ) Total cellular lysates from the DRG of naive mice and recombinant NT3 standard (SinoBiological) are Western blotted with an anti-NT3 antibody (ANT-003, Alomone Labs) to demonstrate that this antibody recognizes both pro-NT3 and NT3. proNT3 (~32 kDa), but not NT3 ( ~ 14.5 kDa), is detected in DRG lysate from naive mice. ( B ) pro-NT3 is highly expressed in the DRG, spinal cord, cortex, cerebellum and brainstem.

Techniques: Microinjection, Control, Two Tailed Test

Journal: EMBO Reports

Article Title: Proneurotrophin-3 contributes to chemotherapy-induced neuropathic pain through TrkC-mediated CCL2 elevation in DRG neurons

doi: 10.1038/s44319-025-00534-1

Figure Lengend Snippet: Data: mean ± SD. ( A , B ) Levels of Nt3 , TrkC , and p75 NTR mRNAs ( A ) and proNT3, TrkC, and p75 proteins ( B ) in the ipsilateral L3/4 DRGs 42 days after microinjection of AAV5-NT3 (NT3) or AAV5-GFP (GFP) into unilateral L3/4 DRGs of the mice with co-microinjection of control scrambled siRNA (Scr-si), TrkC siRNA (TrkC-si), or p75 NTR siRNA (p75-si). n = 3 biological repeats (6 mice)/group. * P < 0.05, ** P < 0.01 by one-way ANOVA followed by post hoc Tukey test. ( C – I ) Paw withdrawal frequencies to 0.07 g ( C , G ) and 0.4 g ( D , H ) von Frey filament stimuli and paw withdrawal latencies to heat ( E, I ) and cold stimuli ( F ) on the ipsilateral ( C – F ) and contralateral ( G – I ) sides on days as indicated after AAV-NT3 or AAV-GFP microinjection in the mice with co-microinjection of control scrambled siRNA (Scr-si), TrkC siRNA (TrkC-si), or p75 NTR siRNA (p75-si) starting at 35 days after AAV5 microinjection. n = 9 mice/group. * P < 0.05, ** P < 0.01 versus the AAV5-NT3 plus control scrambled siRNA-treated group at the corresponding days by two-way ANOVA with repeated measures followed by Tukey post hoc test. ( J ) Levels of p-ERK1/2, total ERK1/2, and GFAP in the ipsilateral L3/4 dorsal horn on day 42 after microinjection of AAV5-NT3 (NT3) or AAV5-GFP (GFP) into unilateral L3/4 DRGs of the mice with co-microinjection of control scrambled siRNA (Scr-si), TrkC siRNA (TrkC-si), or p75 NTR siRNA (p75-si). n = 3 mice/group. * P < 0.05, ** P < 0.01 by one-way ANOVA followed by Tukey post hoc test. .

Article Snippet: Figure EV1 Expression of proNT3, but not NT3, is detected in the DRG of naive and paclitaxel-treated mice. ( A ) Total cellular lysates from the DRG of naive mice and recombinant NT3 standard (SinoBiological) are Western blotted with an anti-NT3 antibody (ANT-003, Alomone Labs) to demonstrate that this antibody recognizes both pro-NT3 and NT3. proNT3 (~32 kDa), but not NT3 ( ~ 14.5 kDa), is detected in DRG lysate from naive mice. ( B ) pro-NT3 is highly expressed in the DRG, spinal cord, cortex, cerebellum and brainstem.

Techniques: Microinjection, Control

Journal: EMBO Reports

Article Title: Proneurotrophin-3 contributes to chemotherapy-induced neuropathic pain through TrkC-mediated CCL2 elevation in DRG neurons

doi: 10.1038/s44319-025-00534-1

Figure Lengend Snippet: Data: mean ± SD. Data: mean ± SD. ( A – C ) Levels of CCL2 and TrkC proteins in the ipsilateral L3/4 DRGs 14 days after the first injection of paclitaxel (PTX) or vehicle (Veh) from the NT3 siRNA- or negative control scrambled siRNA (NC siRNA)-microinjected male ( A ) and female ( B ) mice or 28 days after the first injection of paclitaxel or vehicle from the tamoxifen-treated NT3 f/f mice and NT3 cKD mice ( C ). n = 3 biological repeats (6 mice in ( A , B ); 3 mice in C)/group. * P < 0.05, ** P < 0.01 by two-way ANOVA followed by post hoc Tukey test. ( D ) Level of CCL2 protein in the ipsilateral L3/4 DRGs 42 days after microinjection of AAV5-NT3 (NT3) or AAV5-GFP (GFP) into unilateral L3/4 DRGs of the mice with co-microinjection of control scrambled siRNA (Scr-si), or TrkC siRNA (TrkC-si). n = 3 biological repeats (6 mice)/group. * P < 0.05, ** P < 0.01 by one-way ANOVA followed by post hoc Tukey test. ( E – H ) Levels of Nt3 mRNA ( E , G ), Ccl2 mRNA ( E , G ), proNT3 protein ( F , H ) and CCL2 protein ( F , H ) in the cultured DRG neurons ( E , F ) or in human neuroblastoma cell lines ( G , H ) with transfection of NT3 siRNA or negative control scrambled siRNA (NC siRNA) plus with exposure to paclitaxel (PTX) or vehicle (Veh). n = 3 biological repeats. * P < 0.05, ** P < 0.01 by one-way ANOVA followed by post hoc Tukey test. ( I , J ) Levels of Nt3 mRNA ( I ), Ccl2 mRNA ( I ), proNT3 protein ( J ), and CCL2 protein ( J ) in the cultured DRG neurons with transduction of AAV5-NT3 (NT3) or AAV5-GFP (GFP) plus with transfection of NT3 siRNA or negative control scrambled siRNA (NC siRNA). n = 3 biological repeats. * P < 0.05, ** P < 0.01 by one-way ANOVA followed by post hoc Tukey test. .

Article Snippet: Figure EV1 Expression of proNT3, but not NT3, is detected in the DRG of naive and paclitaxel-treated mice. ( A ) Total cellular lysates from the DRG of naive mice and recombinant NT3 standard (SinoBiological) are Western blotted with an anti-NT3 antibody (ANT-003, Alomone Labs) to demonstrate that this antibody recognizes both pro-NT3 and NT3. proNT3 (~32 kDa), but not NT3 ( ~ 14.5 kDa), is detected in DRG lysate from naive mice. ( B ) pro-NT3 is highly expressed in the DRG, spinal cord, cortex, cerebellum and brainstem.

Techniques: Injection, Negative Control, Microinjection, Control, Cell Culture, Transfection, Transduction

Journal: EMBO Reports

Article Title: Proneurotrophin-3 contributes to chemotherapy-induced neuropathic pain through TrkC-mediated CCL2 elevation in DRG neurons

doi: 10.1038/s44319-025-00534-1

Figure Lengend Snippet: Co-expression analysis of Nt3 mRNA, TrkC mRNA and Ccl2 mRNA in individual large (>35 μm in diameter; ( A ), medium (25–35 µm in diameter; ( B ), and small (<25 μm in diameter; ( C ) DRG neurons from naive mice ( n = 2) by single-cell RT-PCR assay. NeuN mRNA is used as a neuronal marker. Tuba-1α mRNA is used as a loading control. M: DNA ladder marker. NC: no cDNA. n = 5 neurons/size. .

Article Snippet: Figure EV1 Expression of proNT3, but not NT3, is detected in the DRG of naive and paclitaxel-treated mice. ( A ) Total cellular lysates from the DRG of naive mice and recombinant NT3 standard (SinoBiological) are Western blotted with an anti-NT3 antibody (ANT-003, Alomone Labs) to demonstrate that this antibody recognizes both pro-NT3 and NT3. proNT3 (~32 kDa), but not NT3 ( ~ 14.5 kDa), is detected in DRG lysate from naive mice. ( B ) pro-NT3 is highly expressed in the DRG, spinal cord, cortex, cerebellum and brainstem.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Marker, Control